Sulfamethoxazole ELISA Test Kit
RND99017
Product Description
REAGEN™Sulfamethoxazole ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfamethoxazole in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
§ Rapid extraction method for various samples with high recovery (80-105%).
§ High sensitivity (0.5 ppb) and low detection limit (0.25 ppb for shrimp).
§ High reproducibility.
§ A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti- Sulfamethoxazole antibodies. Sulfamethoxazole standards or sample solution, Sulfamethoxazole–HRP conjugate and anti-Sulfamethoxazole antibodies are added. Free Sulfamethoxazole and Sulfamethoxazole-HRP Conjugate compete for the Sulfamethoxazole antibody binding sites. At the same time, the anti- Sulfamethoxazole antibodies are also bound by the immobilized capture antibodies. Any unbound Sulfamethoxazole-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.